WebNov 14, 2014 · pH-Xtra® Glycolysis Assay is a fluorescence-based assay that measures extracellular acidification (ECAR). The assay detects changes in extracellular pH caused by the acid extrusion of either suspension or adherent cells. This allows convenient plate based analysis of glycolytic flux and can be used to monitor alterations in glucose metabolism ... WebThe Lactate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of L-lactate in biological samples. Lactate is produced by glycolysis, a major metabolic pathway responsible for glucose …
Intracellular pH controls WNT downstream of glycolysis in …
WebAug 3, 2024 · The pH-Xtra assay is a TRF-based assay to assess extracellular acidification as a measure of glycolytic activity. Dr Ann-Cathrin Volz (10) Metabolic perturbations play … WebGlycolysis Assay [Extracellular Acidification] (ab197244) is an easy mix-and-measure, 96 or 384 well fluorescence plate reader-based ... Warm to assay temperature (37°C), adjust to pH = 7.4 and filter sterilize using a 0.22 µm filter. Aliquot buffer so that you have enough volume to perform the desired number of assays. Store at - 20°C ... simple club youtube geschichte
pH-Xtra Glycolysis Assay - [PDF Document]
WebThe Agilent pH-Xtra™ Glycolysis Assay allows direct, real-time, kinetic analysis of extracellular acidi-fication rates (ECA/ECAR). BioTek offers a dedicated filter cube … WebScreening of glycolysis inhibitors. Glycolysis Assay Kit - Key Features Fast and sensitive. Use of 5 uL sample. Linear detection range up to 10 mM L-lactate in 96-well plate assay. Convenient. The procedure involves adding … WebJun 24, 2024 · To test this possibility, we incubated recombinant β-catenin protein with acetyl-CoA sodium salt in vitro at pH 5.0, pH 6.3, pH 7.2 and pH 7.6 for 3 h at 37 °C. rawcliffe road goole dn14 8ga